The Djurtus are winless in their last five games in all competitions while Nigeria maintain a five-game unbeaten streak, having started the tournament with back-to-back wins.
According to the latest ranking in December, Guinea-Bissau are 106th in the world and they are 24th on the continent.
Nigeria, on the other hand, are ranked 36th on the global standings and fifth in Africa.
Wednesday's night encounter at the Roumde Adjia Stadium will be the first match between the two countries on the international scene.
The West Africans are without a goal after playing two games in Group D.
Unfortunately for them, Mama Balde thought he had scored an 84th-minute equaliser against Egypt on Saturday but it was ruled out by VAR for an infringement in the build-up.
Guinea-Bissau have not scored a goal since September 2021 when they defeated Sudan 4-2 in a Fifa World Cup qualifying match.
The Djurtus are making their third appearance on the Afcon stage in Cameroon and they have not progressed beyond the group stage in their history.
Meanwhile, the Super Eagles are three-time champions and they are playing in their 19th Afcon tournament.
After winning their first two matches, Nigeria lead Group D with six points after two matches while the Djurtus sit in the third position with just a point which they got after a goalless draw against Sudan on January 11.
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