As Masandawana face what is seen as their toughest group challenge, Mweene starts instead of the injured Denis Onyango who did not concede in the previous two group games.
Mudau keeps his place at right-back as he continues to cement his place as a regular starter for the Brazilians in both domestic and continental assignments.
At left-back, Lakay is expected to get into advantageous positions in the opponent's area.
The Kenyan - who was sidelined by injuries for the most part of the PSL's first-round - has been named again at the centre of Masandwana's defence.
Together with Mandela, the Bafana Bafana centre-back will be tasked to keep a close eye on Al Ahly's attackers led by Mohamed Sherif.
At the centre of midfield, the 25-year-old will have the huge task of frustrating Al Ahly's attacking midfielders during the Saturday clash in Cairo.
His experience will be key as he leads the PSL giants from deep in midfield against the African reigning champions who are under Mamelodi Sundowns' former coach, Pitso Mosimane.
Mamelodi Sundowns' dependable attacking midfielder will face a big challenge as he comes up against Malian Aliou Dieng who has established himself as Al Ahly's strong deep-lying playmaker.
Mokoena replaced Jali in the 87th minute during the game against Al Merrikh.
He was replaced just after the hour mark by Kermit Erasmus - who is on the bench - in their last game but has been trusted with another starting role.
The Namibian has been without a goal in the last four games.
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