Kane scored in the first half of Spurs' Carabao Cup semi-final with Chelsea, calmly slotting home from the penalty spot after being felled by Kepa Arrizabalaga inside the box.
Kane was originally flagged offside, but after a check by VAR the forward was deemed onside and Michael Oliver pointed to the spot.
With the goal, Kane notched his 20th goal of the season, marking the fifth straight campaign where he's hit that mark.
The run began with his 31-goal campaign in 2014-15, which he then followed up on with a 28-goal season one year later.
Kane then fired 28 goals the next season before last campaign brought his best total yet as the Spurs forward fired 41 goals for Spurs in 48 games in 2017-18.
160 - Harry Kane is now the outright fourth highest goalscorer for Tottenham Hotspur. Climbing. pic.twitter.com/Ri9nAnporn— OptaJoe (@OptaJoe) January 8, 2019
In addition, Kane's finish saw him become the fourth top goalscorer in Tottenham history with 160 goals.
The 25-year-old forward trails only Jimmy Greaves, Bobby Smith and Martin Chivers, who scored 266, 208 and 174 goals in their careers, respectively.
Tuesday's goal marked the sixth consecutive match in which Kane has found the back of the net; the second time in his career he has scored in that many matches in a row.